Emerging threat: Antimicrobial resistance proliferation during epidemics - A case study of the SARS-CoV-2 pandemic in South Brazil.

The escalating global concern of antimicrobial resistance poses a significant challenge to public health. This study delved into the occurrence of resistant bacteria and antimicrobial resistance genes in the waters and sediments of urban rivers and correlated this emergence and the heightened use of antimicrobials during the COVID-19 pandemic. Isolating 45 antimicrobial-resistant bacteria across 11 different species, the study identifies prevalent resistance patterns, with ceftriaxone resistance observed in 18 isolates and ciprofloxacin resistance observed in 13 isolates. The detection of extended-spectrum ß-lactamases, carbapenemases, and acquired quinolone resistance genes in all samples underscores the gravity of the situation. Comparison with a pre-pandemic study conducted in the same rivers in 2019 reveals the emergence of previously undetected new resistant species, and the noteworthy presence of new resistant species and alterations in resistance profiles among existing species. Notably, antimicrobial concentrations in rivers increased during the pandemic, contributing significantly to the scenario of antimicrobial resistance observed in these rivers. We underscore the substantial impact of heightened antimicrobial usage during epidemics, such as COVID-19, on resistance in urban rivers. It provides valuable insights into the complex dynamics of antimicrobial resistance in environmental settings and calls for comprehensive approaches to combat this pressing global health issue, safeguarding both public and environmental health.

Pharmaceutical-contaminated irrigation water: implications for ornamental plant production and phytoremediation using enrofloxacin-accumulating species.

Enrofloxacin (Enro) has been widely encountered in natural water sources, and that water is often used for irrigation in crop production systems. Due to its phytotoxicity and accumulation in plant tissues, the presence of Enro in water used for crop irrigation may represent economical and toxicological concerns. Here, we irrigated two ornamental plant species (Zantedeschia rehmannii Engl. and Spathiphyllum wallisii Regel.) with water artificially contaminated with the antimicrobial enrofloxacin (Enro; 0, 5, 10, 100, and 1000 µg L-1) to evaluate its effects on ornamental plant production, as well as its accumulation and distribution among different plant organs (roots, leaves, bulbs, and flower stems), and examined the economic and environmental safety of commercializing plants produced under conditions of pharmaceutical contamination. The presence of Enro in irrigation water was not found to disrupt plant growth (biomass) or flower production. Both species accumulated Enro, with its internal concentrations distributed as the following: roots > leaves > bulbs > flower stems. In addition to plant tolerance, the content of Enro in plant organs indicated that both Z. rehmannii and S. wallisii could be safety produced under Enro-contaminated conditions and would not significantly contribute to contaminant transfer. The high capacity of those plants to accumulate Enro in their tissues, associated with their tolerance to it, indicates them for use in Enro-phytoremediation programs.

COVID-19 pharmaceuticals in aquatic matrices: The threatening effects over cyanobacteria and microalgae.

Water contamination by pharmaceuticals is a global concern due to their potential negative effects on aquatic ecosystems and human health. This study examined the presence of three repositioned drugs used for COVID-19 treatment: azithromycin (AZI), ivermectin (IVE) and hydroxychloroquine (HCQ) in water samples collected from three urban rivers in Curitiba, Brazil, during August and September 2020. We conducted a risk assessment and evaluated the individual (0, 2, 4, 20, 100 and 200 µg.L-1) and combined (mix of the drugs at 2 µg.L-1) effects of the antimicrobials on the cyanobacterium Synechococcus elongatus and microalga Chlorella vulgaris. The liquid chromatography coupled to mass spectrometry results showed that AZI and IVE were present in all collected samples, while HCQ occurred in 78 % of them. In all the studied sites, the concentrations found of AZI (up to 2.85 µg.L-1) and HCQ (up to 2.97 µg.L-1) represent environmental risks for the studied species, while IVE (up to 3.2 µg.L-1) was a risk only for Chlorella vulgaris. The hazard quotients (HQ) indices demonstrated that the microalga was less sensitive to the drugs than the cyanobacteria. HCQ and IVE had the highest values of HQ for the cyanobacteria and microalga, respectively, being the most toxic drugs for each species. Interactive effects of drugs were observed on growth, photosynthesis and antioxidant activity. The treatment with AZI + IVE resulted in cyanobacteria death, while exposure to the mixture of all three drugs led to decreased growth and photosynthesis in the cells. On the other hand, no effect on growth was observed for C. vulgaris, although photosynthesis has been negatively affected by all treatments. The use of AZI, IVE and HCQ for COVID-19 treatment may have generated surface water contamination, which could increased their potential ecotoxicological effects. This raises the need to further investigation into their effects on aquatic ecosystems.

The phytoremediation capacity of Lemna minor prevents deleterious effects of anti-HIV drugs to nontarget organisms.

We investigated physiological responses of Lemna minor plants and their capacity to remove tenofovir (TNF; 412 ng l-1), lamivudine (LMV; 5428 ng l-1) and/or efavirenz (EFV; 4000 ng l-1) from water through phytoremediation. In addition, the toxicological safety of water contaminated with these drugs after treatment with L. minor plants to photosynthetic microorganisms (Synechococcus elongatus and Chlorococcum infusionum) was evaluated. The tested environmental representative concentrations of drugs did not have a toxic effect on L. minor, and their tolerance mechanisms involved an increase in the activity of P450 and antioxidant enzymes (catalase and ascorbate peroxidase). L. minor accumulated significant quantities of TNF, LMV and EFV from the media (>70%), and the interactive effect of LMV and EFV increased EFV uptake by plants submitted to binary or tertiary mixture of drugs. Photosynthetic microorganisms exposed to TNF + LMV + EFV showed toxicological symptoms which were not observed when exposed to contaminated water previously treated with L. minor. An increased H2O2 concentrations but no oxidative damage in S. elongatus cells exposed to non-contaminated water treated with L. minor was observed. Due to its capacity to tolerate and reclaim anti-HIV drugs, L. minor plants must be considered in phytoremediation programs. They constitute a natural-based solution to decrease environmental contamination by anti-HIV drugs and toxicological effects of these pharmaceuticals to nontarget organisms.

Salvinia molesta phytoremediation capacity as a nature-based solution to prevent harmful effects and accumulation of ciprofloxacin in Neotropical catfish.

Phytoremediation has been a potential solution for the removal of pharmaceuticals from water. Here, we evaluated the toxicological safety of ciprofloxacin-contaminated water treated by 96 h with Salvinia molesta. The Neotropical catfish Rhamdia quelen was used as a model, and the potential of the phytoremediation technique for mitigating the drug accumulation in the fishes was also studied. Fish exposed to Cipro (1 and 10 µg·L-1) in untreated water showed toxic responses (alteration of hematological, genotoxicity, biochemical, and histopathological biomarkers) and accumulated Cipro in their muscles at concentrations high for human consumption (target hazardous quotient > 1). Fish exposed to water treated with S. molesta showed no toxic effect and no accumulation of Cipro in their tissues. This must be related to the fact that S. molesta removed up to 97% of Cipro from the water. The decrease in Cipro concentrations after water treatment with S. molesta not only prevented the toxic effects of Cipro on R. quelen fish but also prevented the antimicrobial accumulation in fish flesh, favouring safe consumption by humans. For the very first time, we showed the potential of phytoremediation as an efficiently nature-based solution to prevent environmental toxicological effects of antimicrobials to nontarget organisms such as fish and humans. The use of S. molesta for Cipro-removal from water is a green technology to be considered in the combat against antimicrobial resistance.

Alkylphenols cause cytotoxicity and genotoxicity induced by oxidative stress in RTG-2 cell line.

Alkylphenols ethoxylates are industrial surfactants, and the release in the environmental matrices produces degraded products, of which nonylphenol (NP) and octylphenol (OP) were the most common. They can be classified as endocrine disruptors since the estrogenic potential is widely recognized, but some others toxic aspects are in discussion. This study aimed to evaluate the toxicity of NP, OP, and mixtures of both through cellular, biochemical and genetic biomarkers in fish gonadal cell line RTG-2 exposed to nominal concentrations of 0.05; 0.5; 5; 50, and 100 µg mL-1 of each chemical and their mixtures of 0.05, 0.5; 5 µg mL-1 concentrations. After 24 h, the cells were collected for cytotoxic (neutral red - NR; crystal violet - CV, resazurin assay - RA and lactate-dehydrogenase - LDH), antioxidant system (glutathione-s-transferase - GST; superoxide-dismutase - SOD; glutathione-peroxidase - GPx and malondialdehyde - MDA) and genotoxic assays (alkaline comet assay and Fpg-modified alkaline comet assay). The chemicals and their mixtures were cytotoxic at 50 and 100 µg mL-1, in general aspect, but LDH showed cytotoxicity since 0.05 µg mL-1. The GST and SOD showed an activity increase trend in most tested groups, while GPx decreased at 5 µg mL-1 of the mixture. The MDA increase in all groups resulted in lipid peroxidation. The reactive oxygen species caused DNA damage for all groups. The tested chemicals and concentrations have been found in the freshwater systems. They can induce cell toxicity in several parameters that could impair the gonadal tissues considering the RTG-2 responses.

Physiological responses and phytoremediation capacity of floating and submerged aquatic macrophytes exposed to ciprofloxacin.

Ciprofloxacin (Cipro) water contamination is a global concern, having reached disturbing concentrations and threatening the aquatic ecosystems. We investigated the physiological responses and Cipro-phytoremediation capacity of one floating (Salvinia molesta D.S. Mitchell) and one submerged (Egeria densa Planch.) species of aquatic macrophytes. The plants were exposed to increased concentrations of Cipro (0, 1, 10, and 100 µg.Cipro.L-1) in artificially contaminated water for 96 and 168 h. Although the antibiotic affected the activities of mitochondrial electron transport chain enzymes, the resulting increases in H2O2 concentrations were not associated with oxidative damage or growth reductions, mainly due to the activation of antioxidant systems for both species. In addition to being tolerant to Cipro, after only 96 h, plants were able to reclaim more than 58% of that from the media. The phytoremediation capacity did not differ between the species, however, while S. molesta bioaccumulate, E. densa appears to metabolize Cipro in their tissues. Both macrophytes are indicated for Cipro-phytoremediation projects.

The aquatic macrophyte Salvinia molesta mitigates herbicides (glyphosate and aminomethylphosphonic acid) effects to aquatic invertebrates.

We evaluated the individual and combined effects of different environmentally representative concentrations of glyphosate (0, 25, 50, 75, and 100 µg l-1) and aminomethylphosphonic acid (AMPA; 0, 12.5, 25, 37.5, and 50 µg l-1) on the physiology of Aedes aegypti larvae, as well as the capacity of the aquatic macrophyte Salvinia molesta to attenuate those compounds' toxicological effects. Larvae of Ae. aegypti (between the third and fourth larval stages) were exposed for 48 h to glyphosate and/or AMPA in the presence or absence of S. molesta. Glyphosate and AMPA induced sublethal responses in Ae. aegypti larvae during acute exposures. Plants removed up to 49% of the glyphosate and 25% of AMPA from the water, resulting in the exposure of larvae to lower concentration of those compounds in relation to media without plants. As a result, lesser effects of glyphosate and/or AMPA were observed on larval acetylcholinesterase, P450 reductase, superoxide dismutase, mitochondrial electron transport chain enzymes, respiration rates, and lipid peroxidation. In addition to evidence of deleterious effects by media contamination with glyphosate and AMPA on aquatic invertebrates, our results attest to the ability of S. molesta plants to mitigate the toxicological impacts of those contaminants.

Unique microbiome in organic matter-polluted urban rivers.

Approximately half of the global annual production of wastewater is released untreated into aquatic environments, which results in worldwide organic matter pollution in urban rivers, especially in highly populated developing countries. Nonetheless, information on microbial community assembly and assembly-driving processes in organic matter-polluted urban rivers remains elusive. In this study, a field study based on water and sediment samples collected from 200 organic matter-polluted urban rivers of 82 cities in China and Indonesia is combined with laboratory water-sediment column experiments. Our findings demonstrate a unique microbiome in these urban rivers. Among the community assembly-regulating factors, both organic matter and geographic conditions play major roles in determining prokaryotic and eukaryotic community assemblies, especially regarding the critical role of organic matter in regulating taxonomic composition. Using a dissimilarity-overlap approach, we found universality in the dynamics of water and sediment community assembly in organic matter-polluted urban rivers, which is distinctively different from patterns in eutrophic and oligotrophic waters. The prokaryotic and eukaryotic communities are dominated by deterministic and stochastic processes, respectively. Interestingly, water prokaryotic communities showed a three-phase cyclic succession of the community assembly process before, during, and after organic matter pollution. Our study provides the first large-scale and comprehensive insight into the prokaryotic and eukaryotic community assembly in organic matter-polluted urban rivers and supports their future sustainable management.

Do anti-HIV drugs pose a threat to photosynthetic microorganisms?

We investigated the occurrence and risk assessment of three anti-HIV drugs [(tenofovir (TNF), lamivudine (LMV) and efavirenz (EFV)] in urban rivers from Curitiba (Brazil), as well as the individual and combined effects of their environmental representative concentrations on the freshwater periphytic species Synechococcus elongatus (Cyanobacteria) and Chlorococcum infusionum (Chlorophyta). The three studied drugs, except TNF, were found in 100% of the samples, and concentrations in samples ranged from 165 to 412 ng TNF L-1, 173-874 ng LMV L-1 and 13-1250 ng EFV L-1. Bioassays using artificial contaminated water showed that at environmental concentrations, TNF and LMV did not represent environmental risks to the studied photosynthetic organisms. However, EFV was shown to be toxic, affecting photosynthesis, respiration, and oxidative metabolism. The studied drugs demonstrated interactive effects. Indeed, when submitted to the combination of TNF and LMV, decreased photosynthesis was observed in C. infusionum cells. Moreover, the toxic effects of EFV were amplified in both species when TNF and/or LMV were added to the media. The simultaneous presence of TNF, LMV and EFV in environmental matrices associated with their interactive effects, lead to increased toxicological effects of water contaminated by anti-HIV drugs and thus to an ecological threat to photosynthetic microorganisms.

Sublethal biochemical, histopathological and genotoxicological effects of short-term exposure to ciprofloxacin in catfish Rhamdia quelen.

Ciprofloxacin (Cipro) is commonly detected in water worldwide, however, the ecotoxicological effects to aquatic biota is still not fully understood. In this study, using multiple biomarkers, it was investigated sublethal effects of short-term exposure to Cipro concentrations (1, 10 and 100 µg.L-1) in the Neotropical catfish Rhamdia quelen compared to non-exposure treatment (Control). After 96 h of exposure, the fishes were anesthetized for blood collection to hematological and genotoxicity biomarkers analysis. After euthanasia, the brain and muscle were sampled for biochemical biomarkers analyses. Gills, liver and posterior kidney for genotoxicity, biochemical and histopathological biomarkers analysis and anterior intestine for histopathological biomarkers analysis. Genotoxicity was observed in all tissues, regardless of the Cipro concentrations. Hematological alterations, such as reduction of the number of erythrocytes and leucocytes, as well as in hematocrit concentration and histopathological damages, such as reduction of microridges in gill epithelium and necrosis in liver and posterior kidney, occurred mainly at 100 µg.L-1. In addition, at 100 µg.L-1, Cipro increased antioxidant system activity (Catalase in liver and posterior kidney). These results demonstrated that under short-term exposure, Cipro causes toxic effects in R. quelen that demands attention and surveillance of environmental aquatic concentrations of this antibiotic.

The Role of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Catalase) in the Tolerance of Lemna minor to Antibiotics: Implications for Phytoremediation.

We investigated the individual and combined contributions of two distinct heme proteins namely, ascorbate peroxidase (APX) and catalase (CAT) on the tolerance of Lemna minor plants to antibiotics. For our investigation, we used specific inhibitors of these two H2O2-scavenging enzymes (p-aminophenol, 3-amino,1,2,4-triazole, and salicylic acid). APX activity was central for the tolerance of this aquatic plant to amoxicillin (AMX), whereas CAT activity was important for avoiding oxidative damage when exposed to ciprofloxacin (CIP). Both monitored enzymes had important roles in the tolerance of Lemna minor to erythromycin (ERY). The use of molecular kinetic approaches to detect and increase APX and/or CAT scavenging activities could enhance tolerance, and, therefore, improve the use of L. minor plants to reclaim antibiotics from water bodies.

Evaluating aquatic macrophytes for removing erythromycin from contaminated water: floating or submerged?

Water contamination by antibiotics is an emerging global problem, with impacts on both public health and the environment. Erythromycin has been encountered in bodies of water throughout the world, which demands the development of efficient remediation technologies. We investigated the physiological responses and phytoremediation capacity of four species of aquatic macrophytes, two floating (Salvinia molesta and Lemna minor) and two submerged (Myriophyllum aquaticum and Rotala rotundifolia). The plants were exposed to relevant environmental concentrations of erythromycin (0 and 1.7 µg l-1) in artificially contaminated water for seven days. Physiological evaluations evidenced the ability of that antibiotic to promote oxidative events in those plants, such as the activation of antioxidant enzymes (ascorbate peroxidase and/or catalase). S. molesta exposed to erythromycin demonstrated accumulations of hydrogen peroxide and oxidative damage (lipid peroxidation) that was reflected in growth reductions. The erythromycin removal efficiency of floating plants varied from 9 to 12%, while submerged species varied from 31 to 44%. As such, submerged macrophyte species demonstrated the most efficient removal of erythromycin from contaminated waters, and are therefore more indicated for antibiotic phytoremediation projects.

For the very first time, the capacities of floating and submerged plant species used for removing erythromycin from contaminated water were compared. Moreover, plant physiological responses were related to their phytoremediation capacity. Our results promise to have direct impacts on plant and environmental science as well as in toxicology since they will contribute to a better understanding of the effects of antibiotics in plants and indicate species for better performance of phytoremediation programs aiming to reclaim the antibiotic erythromycin.

Fertilization assures mineral nutrition but does not overcome the effects of Fe accumulation in plants grown in iron ore tailings.

The rupture of Fundão dam was the biggest environmental disaster of the worlds' mining industry, dumping tons of iron ore tailings into the environment. Studies have shown that the Fundão dam's tailings are poor in nutrients and have high Fe and Mn concentration. In this context, our objective was to evaluate the growth performance of two native tree species (Bowdichia virgilioides and Dictyoloma vandellianum) in two treatments: fertilized soil and fertilized tailings. We hypothesize that the high concentrations of iron and manganese in the tailings can impair the growth performance of plants by interfering with the absorption of nutrients made available through fertilization. Soil and tailings samples were collected in the municipality of Barra Longa (MG, Brazil), and then fertilized with mixed mineral fertilizer ("Osmocote Plus 15-9-12" at 7.5 g L-1). The experiment was conducted for 75 days in a greenhouse using 180 cm3 tubes. We evaluate chlorophyll content, maximal PSII quantum yield, root length, shoot length, root:shoot ratio, leaf area, specific leaf area and leaf area ratio, dry mass, macro- and micronutrients concentration in the tissues, and metal translocation factor. Although assuring the adequate levels of the main nutrients to plant growth (N, P, K, Ca, and Mg), the fertilization did not reverse the negative effect of tailing on these species. The high concentration of Fe in the tissues associated with less biomass production, lower plant height, smaller leaf area, bigger specific leaf area, and reduced chlorophyll content indicates a probable phytotoxic effect of iron present in the tailings for D. vandellianum. Our results base further field evaluations and longer experiments, which will facilitate the understanding of the performance of tree species submitted to tailings with fertilization. So far, this study suggests that B. virgilioides are more tolerant to excess Fe from the tailings of Fundão dam than D. vandellianum.

Phytoremediation by ornamental plants: a beautiful and ecological alternative.

Phytoremediation is an eco-friendly and economical technology in which plants are used for the removal of contaminants presents in the urban and rural environment. One of the challenges of the technique is the proper destination of the biomass of plants. In this context, the use of ornamental plants in areas under contamination treatment improves landscape, serving as a tourist option and source of income with high added value. In addition to their high stress tolerance, rapid growth, high biomass production, and good root development, ornamental species are not intended for animal and human food consumption, avoiding the introduction of contaminants into the food web in addition to improving the environments with aesthetic value. Furthermore, ornamental plants provide multiple ecosystem services, and promote human well-being, while contributing to the conservation of biodiversity. In this review, we summarized the main uses of ornamental plants in phytoremediation of contaminated soil, air, and water. We discuss the potential use of ornamental plants in constructed buffer strips aiming to mitigate the contamination of agricultural lands occurring in the vicinity of sources of contaminants. Moreover, we underlie the ecological and health benefits of the use of ornamental plants in urban and rural landscape projects. This study is expected to draw attention to a promising decontamination technology combined with the beautification of urban and rural areas as well as a possible alternative source of income and diversification in horticultural production.

Integrative signaling of hydrogen peroxide and gibberellin on Zn-mediated alleviation of thermodormancy in sorghum seeds.

Increasing global temperatures could result in decreasing crop production by decreasing seed germination in the field due to thermodormancy acquisition. Certain metals appear to modulate seed thermodormancy, although the exact mechanisms of that effect have not yet been elucidated. We report here the effects of Zn on the thermodormancy of sorghum seeds. Seeds treated with 0 or 200 mg Zn L-1 were germinated at optimal (30°C) and supra-optimal (40°C) temperatures and their germinability and oxidative stress markers were evaluated. The integrative effects of Zn, abscisic acid (ABA), gibberellin (GA), and H2 O2 on the physiology of seed thermodormancy were examined. The supra-optimal germination temperature (40°C) induced seed thermodormancy, which was, however, alleviated by treatment with 200 mg Zn L-1 . Thermodormancy acquired at supra-optimal temperatures in sorghum seeds must reflect de novo synthesis and accumulation of ABA. Although Zn treatment did not prevent ABA accumulation, it increased the activities of mitochondrial ETC enzymes and decreased the antioxidant enzymes' activity, leading to the accumulation of H2 O2 . By increasing mitochondria activity and H2 O2 production, Zn may induce GA synthesis and alleviate thermodormancy in sorghum seeds. The pretreatment of sorghum seeds with Zn may therefore improve seed germination and assure increased crop performance under normal (30°C) or rising (up to 40°C) temperatures.

Periphytic Algae and Cyanobacteria from the Rio Doce Basin Respond Differently to Metals and Salinity, Showing Different Potential for Bioremediation.

We have studied the isolated and combined effects of metals (Fe and Mn) and NaCl the on growth, physiology, and metal-uptake capacity of two photosynthetic periphytic species-Synechococcus elongatus (Cyanobacteria) and Chlorococcum infusionum (Chlorophyta)-isolated from an impacted area of the Rio Doce River (Brazil) after the Fundão dam collapse. The effective concentrations found to reduce 10 and 50% growth were 15.2 and 31.6 mg Fe L-1, and 2.5 and 7.9 mg Mn L-1 for S. elongatus and 53.9 and 61.6 mg Fe L-1, and 53.2 and 60.9 mg Mn L-1 for C. infusionum. Although the metal toxicity was related to oxidative stress, both species showed activation of antioxidant systems under phytotoxic concentrations of Fe and Mn. By binding large concentrations of metals on its cell surface and thus avoiding their entrance into the cells, C. infusionum presents greater resistance to Fe and Mn than S. elongatus. Under environmental realistic concentrations of Fe and Mn in river water from the Rio Doce Basin, S. elongatus and C. infusionum showed a metal removal efficiency of 42 and 65% and 53 and 79%, respectively after 96 h. These species were insensitive to increased NaCl concentrations which, in addition, did not disrupt the metal removal capacity of the species. Due to their salt and metal tolerance, S. elongatus and C. infusionum can be used for the remediation of waters contaminated with Fe and Mn.

Isolated and combined effects of glyphosate and its by-product aminomethylphosphonic acid on the physiology and water remediation capacity of Salvinia molesta.

We evaluate the isolated and combined effects of glyphosate and its by-product aminomethylphosphonic acid (AMPA) and the potential of the aquatic macrophyte Salvinia molesta to remove these chemicals from contaminated water. Plants were exposed to environmentally relevant concentrations of glyphosate (0, 20, 40, 60, 80 and 100 µg l-1) or AMPA (0, 10, 20, 30, 40 and 50 µg l-1) for seven days. Then, based on the effective concentrations of glyphosate found to reduce photosynthetic rates by 10% (EC10) and 50% (EC50), the plants were exposed to combinations of 0, 16 and 63.5 µg glyphosate l-1 and 0, 5, 15, 25 µg AMPA l-1. The EC(10) and EC(50) were lower for AMPA (6.1 µg l-1 and 28.4 µg l-1 respectively) than for glyphosate (16 and 63.5 µg glyphosate l-1 respectively). When occurring together, the deleterious effects of those chemicals to plants increased. S. molesta plants removed up to 74.15% of glyphosate and 71.34% of AMPA from culture water. Due to its high removal efficiency, S. molesta can be used in phytoremediation programs. It will be important to evaluate the combined effects of glyphosate and AMPA in any toxicological studies of the herbicide.